Eco-friendly analytical methods for the determination of compounds with disparate spectral overlapping: application to antiviral formulation of sofosbuvir and velpatasvir

Abstract Green analytical chemistry is one of the newest trends in nowadays targeting concept green laboratory practices on chemists and environment. In this text, are proposed work for determination sofosbuvir (SF) velpatasvir (VP) their pharmaceutical formulation. The analysis SF a binary mixture with VP represents an challenge due to complete overlapping UV spectrum by that VP. Therefore, direct absorbance derivative measurements cannot resolve such interference failed determine SF. paper, three simple methods were developed without any from sample pre-treatment. include measuring second amplitude ratio using as divisor, difference NaOH solution against its HCl solution, compensation technique. On other hand, was determined specifically presence two methods. Firstly, reaction 4-chloro-7-nitrobenzofurazan (NBD-Cl) where product measured spectrophotometrically spectrofluorometrically secondly through 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH). calibration curves showed good correlation coefficient ( r 2 > 0.999). highly precise RSD% values less than 2%. method greenness profile compared published applying eco-scale protocol. Assessment results proved our procedure greener reported Moreover, upon comparison methods, better or comparable sensitivity addition being inexpensive ecofriendly. Accordingly, these could be readily applied quality control purposes eco-friendly, efficient tool.